Glassware Washers

Glassware maintenance basics:

For your beakers, flasks, funnels

1. First rinse them with an organic solvent like acetone or ethanol. This is to get rid of any organic residue.
2. Throw away the rinse -- it goes into your organic waste.
3. Then scrub the inside with warm soapy water and a brush. This water is okay to go down the sink.
4. Rinse off the soap with deionized water to avoid those pesky water stains. And if your glass isn't gleaming by now, give it another scrub.
5. Lastly, a rinse with acetone should speed up drying. The leftover acetone? It goes with your organic waste, too.

Glassware Washer Detergents

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Burets:

Dismantle them first, then clean the buret and stopcock or rubber tip separately with detergent and water. Rinse until squeaky clean, then with distilled water, and finally let them dry. Before putting the glass stopcock back, make sure to use some lubricant. And remember to cover your burets when you're not using them.

Culture Tubes:

If these guys were used before, sterilize them first. Autoclaving is your best friend here. Hot tubes can be emptied easily if media solidifies when cooling down. Afterwards, it's cleaning time - scrub with detergent and water, rinse with tap water, then distilled water, then let them dry. If you're filling them with media that needs sterilizing, don't plug them until the media is in. That way you can do one round of autoclaving.

Dishes and Culture Bottles:

These follow the same cleaning routine as Culture Tubes. Wrap them up for sterilization in an autoclave or dry air sterilizer.

Pipettes:

After use, put them tips down in a jar of water, being gentle to avoid damaging the tips. Then give them a good soak in dissolved detergent or a chromic acid cleaning solution if they're super dirty. Rinse with tap water until they're clean, then soak in distilled water. Dry them and store them in a dust-free drawer. If you're working with infectious material, plug the pipette with cotton before sterilizing.

Serological Tubes:

These need to be squeaky clean but not necessarily sterile. For tubes with blood, dump the clots, boil them with soap or detergent, rinse, brush, rinse, and dry. Make sure to remove all acids, alkalis, and detergents. Keep these separate from other glassware.

Slides and Cover Glass:

Cleanliness and a scratch-free surface are vital. Wash them, dip them in glacial acetic acid, rinse with distilled water, and wipe them dry. You can store them in a jar of alcohol and dry them as needed. If they're dry-stored, rinse them with alcohol before using.

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